Abstract: Using common combinations of carbon and nitrogen sources,this paper investigated the regulatory effects of key parameters on the selenium-enriched liquid culture of Morchella through single-factor and orthogonal experiments,and concluded the key technical parameters. Through targeted selenium-enriched culture,a composite product with both fungal nutrients and selenium bioactivity can be obtained. The research results have broad market potential in the fields of functional foods,dietary supplements,cosmetic raw materials,and innovative drug development.

Key words: Morchella, selenium-enriched fermentation, selenium polysaccharide, process optimization