T-2毒素神经毒性作用机制研究

天津科技 ›› 2023, Vol. 50 ›› Issue (Z): 18-23.

T-2毒素神经毒性作用机制研究

李道稳^1,2, 刘芳¹, 李源¹, 胡鹏程¹, 刘鼎阔^1,*

1.天津市生物饲料添加剂企业重点实验室,鼎正新兴生物技术（天津）有限公司天津 300383;
2.天津农学院动物科学与动物医学学院天津 300384

收稿日期:2023-09-21 出版日期:2023-12-06 发布日期:2023-12-27
通讯作者: *
基金资助:
国家自然科学基金“基于SIRT1-TFEB轴调控自噬—细胞焦亡通路探讨芍药苷靶向干预氧化锌纳米颗粒肝毒性的作用机制”（32302926）; 中国博士后科学基金“基于数字化分析的饲料霉菌毒素快速检测及生物脱霉剂开发与应用研究”（2021M700708）

Study on Molecular Mechanism of T-2 Toxin-induced Neurotoxicity

LI Daowen^1,2, LIU Fang¹, LI Yuan¹, HU Pengcheng¹, LIU Dingkuo^1,*

1. Tianjin Key Laboratory of Biological Feed Additive Enterprise,S&E Burgeoning Biotechnology <Tianjin> Co.,Ltd.,Tianjin 300383,China;
2. College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300384,China

Received:2023-09-21 Online:2023-12-06 Published:2023-12-27

摘要/Abstract

摘要： T-2毒素是一种具有神经毒性的霉菌毒素,主要污染农作物及其制品,已经发现PC12细胞对低于12 ng/mL的T-2毒素很敏感,表现为细胞活力下降、乳酸脱氢酶（LDH）释放增加并伴有氧化应激和线粒体损伤、Nrf2/HO-1通路上调、线粒体膜电位（MMP）降低和线粒体凋亡。通过研究,首次发现T-2毒素可引发神经炎症,表现为NF-κB的表达和易位增加,以及提高促炎细胞因子TNF-α、IL-6、IL-8和IL-1β的分泌。综上所述,T-2毒素刺激PC12细胞诱发细胞凋亡和炎症,而氧化应激在神经毒性过程中起关键作用。

关键词: T-2毒素, 神经毒性, 神经炎症, 氧化应激, 细胞凋亡

Abstract: T-2 toxin is a neurotoxic mycotoxin,which mainly pollutes crops and their products. PC12 cells have been found to sensitive to T-2 toxins lower than 12 ng/mL,which was manifested as decreased cell viability,increased lactate dehydrogenase （LDH） release accompanied by oxidative stress and mitochondrial damage,and up-regulated Nrf2/HO-1 pathway,inducing decreased mitochondrial membrane potential （MMP） and mitochondrial apoptosis. For the first time,it was found that T-2 toxins could trigger neuroinflammation,manifested as increased expression and translocation of NF-κB and increased secretion of pro-inflammatory cytokines TNF-α,IL-6,IL-8 and IL-1β. In summary,T-2 toxins stimulate PC12 cells to induce apoptosis and inflammation,while oxidative stress plays a key role in the neurotoxic process.

Key words: T-2 toxin, neurotoxicity, neuroinflammation, oxidative stress, apoptosis

中图分类号:

S859.83

李道稳, 刘芳, 李源, 胡鹏程, 刘鼎阔. T-2毒素神经毒性作用机制研究[J]. 天津科技, 2023, 50(Z): 18-23.

LI Daowen, LIU Fang, LI Yuan, HU Pengcheng, LIU Dingkuo. Study on Molecular Mechanism of T-2 Toxin-induced Neurotoxicity[J]. TIANJIN SCIENCE & TECHNOLOGY, 2023, 50(Z): 18-23.

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